eptors 9 of 20 > mRN A tor IPs receptors that do not allow Ca release is injecte

Question

eptors 9 of 20 > mRN A tor IPs receptors that do not allow Ca release is injected, and then the egg is fertilized If these receptors were made in small quantities, they would Dcompete Dwith normal receptors for available calcum. The result would be increased calcium release from the ER tollowing fertilization Ithese receptors were made in suficient quantities, they would Dcompete Dwith normal receptors for available IP, The result would be reduced calcium release from the ER tollowing fertilization calcium release from the Golgi complex following fertlization calcium release from the Golgi complex following fertblization If these receptors were made in sufficient quanities, they would CcompeteD with normal receptors for available calcium The result would be reduced If these receptors were made in suficient quantities, they would Dcompete Owith normal receptors for available IPs The result would be increased Submit Part C Fluo3, a molecule that fluoresces in response to elevated Ca+ levels, is injected into normal eggs, which are then fertilizesd Aequorin fluorescence would only be observed at the site of sperm entry into the ego A wave of GFP aequorin fuorescence would be observed to sweep across the eg9 nitiating at the site of sperm entry into the ega. Little tuorescence would be observed at the site of sperm entry into the ego A wave of aequor in fluore scence would be observed to sweep across the epg, initiating at the site of sperm entry into the egg

Explanation / Answer

1) Calcium ions plays a very important role in many cellular processes and are considered as second messengers of the cell. When mRNA for IP3 receptors that do not allow calcium release is injected and then the egg is fertilised, the modified IP3 receptors would compete with normal receptors for available IP3. The result would be reduced calcium release from the ER following fertilisation (option 2).

2) Fluo3 is a molecule that fluoresces in response to elevated calcium levels. When Fluo3 is injected into normal eggs which are then fertilised a wave of GFP aeqorin fluoresence would be observed to sweep across the egg initiating at the site of sperm entry into the egg. (option 2).

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