64. One copy of the human genome is 3 billion (3 x 10) base pairs of DN.A) Suppo
ID: 270547 • Letter: 6
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64. One copy of the human genome is 3 billion (3 x 10) base pairs of DN.A) Suppose you cut a sample of your genomic DNA to completion with Hinmd Il, a restriction enzyme that recognizes the DNA site 5%.AA GCTT Each copy of your genome is cut into about fragments of average size bp. A) 12,000,000/256 B) 4096/700,000 C) 500,000,000 6 D) 43,000 /65,536 E) 700,000/4096 65. Which of the following steps are unnecessary when generating a homozygous knockout mouse using ES cells? 1. Introduce chimeric host blastocyst into a surrogate mother mouse. 2 Cross chimeric mouse to a normal mouse to obtain a heterozygous knockout mouse 3. To cither end of a drug resistance marker, ligate genomic DNA corresponding to the ends of the gene to be knocked out. 4. Add LTRs to the ends of a cDNA for the gene to be knocked out 5. Introduce your DNA construct into host blastocysts. 6. Introduce heterozygous knockout ES cells to a host blastocyst 7. Mix your ES cells with ES cells conuining a Cre gene. 8. Introduce your DNA construct to ES cells growing in a petri dish. 9. Apply selection for drug resistance marker 10. Introduce your DNA construct to packaging cells. 11. Replace the viral genes with a therapeutic gene. 12. Cross two heterozygous knockout mice to each other to generate a homozygous knockout A) 2,3,9 C) 3, 6, 9, 12 D) 4, 7, 10, 11 E) None of the aboves all of the steps are necessaryExplanation / Answer
Answer 65. Option e
All the steps from 1 to 12 are necessary to produce the knock out mice. The first knock out mice was prepared by Capeecchi, Martin and Smithies in the year 1989, and they were awarded Nobel Prize for it in the year 2007.
For preparing the knock out mice, the original genes of the mice are knocked out and the gene of interest is inserted. After following all the steps the homozygous knock out mice is prepared. They are quite useful for the experimentation on various physiological processes in the human beings, because our general physiology matches to the mice. Such type of mice can be helpful for many amateur discoveries.
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