Case B: LL is a 12-year-old female presenting to her pediatrician, complaining o

Question

Case B: LL is a 12-year-old female presenting to her pediatrician, complaining of sore throat and cough. She has had some hoarseness in her voice over the past few days and subjective sweats but no documented fever. She has a history of seasonal allergies in the fall, and takes loratidine (antiallergic) only during that season. Upon review of systems, she complains of isolated throat pain, without any running nose, sinus pressure, or headache. Her mother has been taking her temperature at home, and they have fluctuated from 97.8°F-99.2°F. Throat examination showed yellow pus dots in the back. Rapid Strep Antigen (RSA) Throat Culture Negative Pending 1. Which is the most likely pathogen causing this infecting? (1pt) 2. What is the most likely reason for which the RSA was negative? (1pt) What do you expect to observe in the throat culture that indicates the likely presence of this pathogen? (1pt) 3. 4. Which culture conditions are most appropriate to culture this microorganism? (1pt)

Explanation / Answer

1. Streptococcal pharyngitis

2. One third of negative rapid strep test are false that is someone actually has a strep infection even though rapid strep results show negative.

5% - 20% of individuals carry strep in their throat without symptomatic information, so the presence of Group A streptococcus in an individual with pharyngitis does not prove that this organism is responsible for the infection.

3. Cocci bacteria( arranged in lines observed under microscope) are observed on blood agar plate. It is regarded as gold standard for treating. Bacteria produces large zones of beta hemolysis that is complete disruption of erythrocytes and release of hemoglobin

4. Trypticase soya agar with 5% sheep blood

Incubate at 370C overnight.

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