Please show how to calculate the total yield. Unlike the example used in #5, you

Question

Please show how to calculate the total yield. Unlike the example used in #5, your DNA sample from Lab 2 did not need to be diluted and its A26o absorbance was measured as is (dilution factor 1). What was the A260 OD measurement (provided by your TA or on Laulima) of your extraction? Calculate the concentration (ug/ml) and total yield of dsDNA of your extraction using the methods described above. Show your work and include the correct units. (4 points) 7. 4uo OD of my extraction [0.92/ ds DM4 concentration 5/ml 0. te.o5/m

Explanation / Answer

The given information is

Absorbance at 260nm of dsDNA = 0.921

dilution factor = 1

1 OD at at 260 nm for pure dsDNA is 50ug/ml

Concentration of dsDNA (ug/ml) = Absorbance * dilution factor * 50ug/ml

Concentration of dsDNA (ug/ml) = 0.921 * 1* 50ug/ml

Concentration of dsDNA (ug/ml) = 46.05ug/ml

Total DNA yield (ug) = DNA concentration * total sample volume (mL)

If the total sample volume is 1 ml

Then the yield is 46.05ug/ml*1mL

Total DNA yield (ug) = 46.05ug

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