Diresctions: This lab will be collected and graded. Complete all sections below.

Question

Diresctions: This lab will be collected and graded. Complete all sections below. Your group will choose potato cells for this molecule. In other words, at what concentra surrounding solution isotonic to the cytoplasm of the potato cels: nicity of one of the 6 biological substances above to determine the to for this molecule. In other words, at what concentration of this molecule is the group, choose one of the following 6 solutions of a biological molecule tound in cells, described above: urea, glycerol, NaCI, glycine, sucrose Our group will use different concentrations of a solution of Coa3 2 )YOU MUST WRITE YOUR HYPOTHESIS FOR WHAT WILL OCCUR ING THE EXPERIMENT AND PREDICTION BEFORE YOU START THE EXER IMENT. Hint: Think about what should happen at concentration or isotonic point. concentrations different than the isotonic 3) Based on your hypothesis, write a prediction. Remember a prediction is written as an "If...., then.... statement. Experiment: at've wate You will have a 1M stock solution that you will dilute to make the concentrations in the chart below. For the experiment you will need to make 100 ml of the diluted solution. CV-CV TABLE 1: Fill out the chart below. Compare with other members of your group to be sure your Final Concentration of Amount (Vi) of calculations agree, Have your instructor check the calculations of one member of your group. Amount of Water Final Volume of the 1 M Stock Solution (V2-V) Solution (V2 Solution (C2) 0 M 0.1 M 0.3 M 0.5 M I M 10 127

Explanation / Answer

hypothesis- if the solution is hypertonic than the cell present in the solution will shrink as water will come out of the cell and if the solution is hypotonic than the cell present in the solution will swell as water will come inside the cell.

for 0 M glucose solution - add 100 ml of water.

for 0.1 M -

1* X = 0.1 *100

X = 10 ml of 1 M glucose solution + 90 ml of water.

for 0.3 M -

1* X = 0.3 *100

X = 30 ml of 1 M glucose solution + 70 ml of water.

for 0.5 M -

1* X = 0.5 *100

X = 50 ml of 1 M glucose solution + 50 ml of water.

for 1 M -

add 100 ml of 1M glucose solution.

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