Besides the method used by V. cholerae , other types of gene transfer exist. One

Question

Besides the method used by V. cholerae, other types of gene transfer exist. One important type is transduction, which can be generalized or specialized. Choose whether the following examples illustrate generalized transduction, specialized transduction, neither or both.

a. Transduced DNA is integrated into the host as part of a prophage

b. it is accomplished by transducing particles

c. only specifc regions of DNA may be transduced

d. A relatively low percentage of cells exposed undergo transduction

e. transduction must involve a temperate phage

f. it is not necessary for phages to be defective for transduction

g. any part of the host genome can be transferred

h. it involves a pilus

i. transduction may involve a virulent phage

Explanation / Answer

Transduction: Transfer of foreign DNA into a cell by a virus or a viral vector.

Generalized transduction: a process by which a bacterial DNA is transferred to another bacterium via a bacteriophage, where a very small amount of phage particles carry a donor bacterium's DNA.

Specialized transduction: a process in which a restricted set of bacterial genes are transferred to another bacterium depending on where the phage genome is located on the chromosome.

a. Specialized transduction. The viral DNA integrates with bacterial DNA and survives in prophage stage within the bacteria for several generations.

b. Both. it is accomplished by transducing virus particles

c. Specialized transduction. Restricted regions of DNA may be transduced

d. Generalized transduction. A relatively low percentage of cells exposed undergo transduction

e. Specialized transduction. transduction must involve a temperate phage

f. Both. it is not necessary for phages to be defective for transduction

g. Generalized transduction. any part of the host genome can be transferred

h. Neither. it involves a pilus

i. Generalized transduction. transduction may involve a virulent phage

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