Assuming you\'ve designed three sets of primers (A, B, and C) for this project.

Question

Assuming you've designed three sets of primers (A, B, and C) for this project. You amplify the DNA fragments on four individual samples using the same PCR protocol. Each PCR consists of only one set of primers. Below are the gel images for each primer set. Primer A Primer B PrimerC 1. i) Which of these primer sets would you choose for your project? a. Primer A b. Primer B c. Primer C d. Primer A and B ii) Why do you choose this/these primer(s)? 2. Why primer C isn't an appropriate marker? 3. i) List two possible reasons why primer A produces an extra band compare to the other primers ii) If you redo the PCR, what would you change in order to determine if the extra band in primer A is a real DNA amplification or it is caused by artificial error?

Explanation / Answer

1. Primer B

Because they are using set of primer the band in primer B shows. Pairs of primer in all of the 4 samples.

2. Primer C is not a marker because all primer accumulated at one point.

3. Primer A has either forward or reverse primer.

ii. Use both the primer to check the result.

4. Primer A = Image 1
Primer B = Image 2

5. Step 1 = Denaturing at 94oC, Image 3
Step 2 = Annealing at 55oC, Image 1
Step 3 = Extending at 72oC, Image 2

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