ination IV Eva Datel 12U 20/2016 BIOL 261-0001 Fall 2016 Dr. Belinet Answer all
ID: 300015 • Letter: I
Question
ination IV Eva Datel 12U 20/2016 BIOL 261-0001 Fall 2016 Dr. Belinet Answer all the following questions (so)on the answer sheet provided. Please return questions and sheets. Time allowedi 1 hour Fill in the blanks 1-3) identification of dhe following streptococci? What test(s) would you use for the presumptive All group D streptococci 3, Group C streptococci Fill in the blanks (4-18 Ords: orows behavior of the medically important suapbodogossi in by CH); epidermidis soprophyrious the following tests Alpha toxin 4 (acid only) 5 coagulase 12 Biotin growth True or False (19-21) characteristics but differ in their antigenic 19. Serovars are strain that have similar biochemical 20. Some Ecoli strains produce toxins that are similar to the cholera toxin 21. The Shigella infect only primate hosts Enrichment media most frequently used to favor the growth of Salmonella and Shigella? 24. mannitol salt agar 22. Selenite F 23. Gram negative broth media enteric agar, and Xylose Lysine Desoxycholate agar are differential because they all contain a dye 27. mannitol and a dye 25. lactose and a dye 26. glucose and In MacConkey agar, coliform colonies are 29. yellow 30. greenish-blue 31. red 28. orange In Hektoen enteric Salmonella and Shigella colonies are agar, 34. greenish-blue 32. orange 33. yellow 35. red In Xylose Lysine agar, Coliform colonies are 36, orange 37. yellow 38. greenish-blue 39. red In xylose Lysine Desoxycholate agar, salmonella and Shigella colonies are 40, orange 41. yellow 42. greenish-blue 43. red. True or False (44-50)Explanation / Answer
1. All group D streptococci can be differentiated by Bile Esculin test. They are all positive for Bile Esculin test. They cannot grow in 6.5% NaCl containing media.
2.S.pyogenes - beta hemolysis on blood agar, sensitive to bacitracin and penicillin, catalse negative, PYR test positive
3.Group C streptococci - Ribose and trehalose fermentation.
4. Alpha toxin - S.aureus Positive
5.Mannitol - S.aureus - Positive
6. Coagulase - S.aureus - Positive
7.Biotin for Growth - S.aureus - Positive
8. Novobiocin- S.aureus - Sensitive.
9. Alpha toxin - S.epidermidis - Negative
10. Mannitol - S.epidermidis - Negative
11.Coagulase - S.epidermidis - Negative
12.Biotin - S.epidermidis - Negative
13.Novobiocin - S.epidermidis - Sensitive
14. Alpha toxin - S.saprophyticus - Negative
15.Mannitol - S.saprophyticus - Negative
16.Coagulase - S.saprophyticus - Negative
17Biotin S.saprophyticus - Negative
18.Novobiocin S.saprophyticus - Resistant
19. True.
Serovars or serotypes of bacteria are classified together based on their cell surface antigens, allowing the epidemiologic classification of organisms to the sub-species level.
20. True. Ecoli produces Enterotoxin which resembles cholera toxin in mode of action but is less severe. Enterotoxigenic E.coli produces this toxin and causes travellers diarrhea.
21. True. Shigella causes bacillary dysentry. It is seen only in primates. Other animals can be experimentally infected in the lab.
Answered 21 of 40 questions. please post the other questions separately in sets of 2 or 3 for answers.
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