Which one? You are setting up an enrichment culture to look for methylotrophic b

Question

Which one?
You are setting up an enrichment culture to look for methylotrophic bacteria in a sample of water from Lake Wellington in Seattle. Methylotrophs are capable of obtaining their carbon and energy needs from single carbon compounds such as methanol. In your enrichment culture, you provide methanol and a variety of macro- and micro-nutrients to encourage growth. However, you must be careful NOT to add which of the following if you are to successfully enrich for methylotrophs? Iron A source of nitrogen Potassium Any other carbon source A source of phosphorous

Explanation / Answer

The Answer is

- Any other carbon source

Explanation:

Since you want to only methylotrophs to grow, methanol should be the only carbon source in the media. If any other carbon source is added then even non-methylotrophic organisms will grow in the media and your media will not be selective for methylotrophs.

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