Control Cdc20 SiRNA Cdo20 Actin 2 18 hr in vitro culture Coa20 is a pratein that

Question

Control Cdc20 SiRNA Cdo20 Actin 2 18 hr in vitro culture Coa20 is a pratein that cells (contro, HeLa cells transfected with Cdc20 siRNA, and a Cdc20 mutant Hela cell line. Blots were stained for Cdc20, Securin, and actin tas a loading control), (B) Embryas from bath wild-type control mice and from mice transfected with Cdo20 s RNA were cultured in vitro cally interacts with complex (APC) to regulate its activity. (A) Western blot analysis of Hela for 18 haurs. Pha left panel) and 18 hours atter incubation (right panel). Astenisks indicate embrycs with Cdc20 sIRNA and all ather embryos are from wild-type oontrol mice. (C) Fluorescence images of fluorescence marker (to outline the cells; red). (D) Histolegy sections obtained from two different individuals with breast cancer. Tissue is labelled for nualei (blue) and seaurin (brown). images were captured immediately following the addition of the embryos at the two cell stage to the culture dish cultured in panel B. Embryps were stained using DAPI DNA marker, blue) and a whale-cell 1. 2. 3. What effect does Cdc20 siRNA have on APC activity? Explain. Which phase of mitosis is altered by Cdc20 siRNA? Of the two histological images of breast cancer presented in panel D, which image might represent someone with a more aggressive form (e.g. more deadly) of breast cancer? Explain. 4. In theory, what effects might the Cdc20 mutant have on cell division?

Explanation / Answer

1. Cdc20 or cell division cycle protein 20 plays important role in regulating cell cycle. Cdc20 used for the activation of APC or anaphase promoting complex. This will result in separation of chromatid and so thus cells can go into anaphase state. Thus cell with siRNA of Cdc20 will lack APC activity and will remain in metaphase state.

2. The transition from metaphase to anaphase will be affected by Cdc20 siRNA

3. Securin is essential for metaphase to anaphase transition. Figure 2 represent more aggresive form of breast cancer, as here securin is overexpressed. Securin regulation is necessary for separation of sister chromatid, and in cancer the amount of abnormal DNA conent increases which also shows increased securin amount.

4. Cdc20 is essential for two function in cell division. During cell division it regulates nuclear movement before anaphase step and also separation of chromosome, So cells with mutation in Cdc20 will have inhibition at metaphase to anaphase progress.

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