01. Fill in all the blanks to balance the following equation for the overall rea
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01. Fill in all the blanks to balance the following equation for the overall reaction of glucose catabolism. 02A. The oxygen we breath in ends up in what molecule? 02B. The carbon in the CO2 we breath out came from what molecule? 03. Complete the following table, assuming a single glucose molecule is catabolized. For location in cell, s pecify pr the enzymes/proteins that perform each step are found. # ATP produced (net gain) Step Aerobic or anaerobic Location in cell # carbons lost # hydrogens bound as Co2(specify for each coenzyme Glycolysis Cytssol Linking step Krebs cycle mati of aurobic a arp Oxidative phosphorylation 04. How much ATP is needed to keep us alive? To answer this question, one first needs an estimate of the energy conter ATP Each mole of ATP would, if broken into ADP and Pi under typical physiological conditions, yield about 12 kilocalorie ergy. And from the balanced equation above, we know how many ATP are made from each glucose that is catabolize Review all the preceeding information by filling in the following blanks: 1 mole of glucose will produce Therefore, the cellular catabolism of 1 mole of glucose will store _34 kcal of energy in the bonds of ATP moles of ATP. And, each mole of ATP contains- I-_ kcal of energyExplanation / Answer
3. I have explained the details one by one...
GLYCOLYSIS.
Cytoplasm
none lost as CO2, 3 carbon atoms end product pyruvate or lactate is produced.
one hydrogen bound to NAD.NAD is the coenzyme.
Both aerobic and anaerobic. In aerobic condition conversion of glucose to pyruvate occur in anaerobic condition pyruvate reduced to lactate. Lactate is regarded as dead end of glycolysis.
In aerobic net gain of 7ATP. In anaerobic glycolysis net gain of 2ATP
LINKING STEP
PDH COMPLEX, Pyruvate dehydrogenase complex located in mitochondrial matrix
One CO2 lost when conversion of pyruvate to acetyl coA occurs.
One hydrogen bound with NAD as coenzyme
Both aerobic and anaerobic. In aerobic process oxidative decarboxylation of pyruvate to acetyl CoA occurs. In anaerobic conditions reduction of pyruvate to lactate occur.
Net gain of 2.5 ATP occur when NADH is oxidized by electron transport chain. As one molecule of glucose produces 2 molecules of pyruvate through glycolysis there is NET SYNTHESIS OF 5 ATP molecules through this reaction per molecule of glucose.
KREBS CYCLE
Mitochondrial matrix, only succinate dehydrogenase found in inner mitochondrial membrane
CO2 lost at three steps in Krebs.
1.conversion of isocitrate to alpha ketoglutarate by isocitrate dehydrogenase
2.conversion of alpha keto glutarate to succinyl CoA by alpha ketoglutarate dehydrogenase
3.conversion of Lmalate to oxaloacetate by malate dehydrogenase.
Three Hydrogens bound with NAD. NAD as coenzyme
Aerobic process
Net gain of 20ATP
OXIDATIVE PHOSPORYLATION : The phosporylation of ADP into ATP Is coupled with oxidation of reducing equivalents, therefore called oxidative phosporylation.
takes place in inner mitochondrial membrane. Electron transport chain
None lost as CO2
Hydrogen bound with NADH and FADH2.
Aerobic
Net gain of ATP by one molecule of glucose by oxidative phosporylation is 32 ATP
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