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Compare and Contrast Southern and Northern blots. a specific DNA sequence with a

ID: 36052 • Letter: C

Question

Compare and Contrast Southern and Northern blots. a specific DNA sequence with a DNA pro be is detected you can detect the amount of a certain type of mRNA and can compare that to another sample produced under different experimental conditions a specific mRNA sequence with a p robe is detected J digest the genomic DNA with a restriction enzyme, separate the DNA fragments by gel electrophoresis, transfer the DNA to a positively charged membrane, and detect a fragment of DNA that contains your DNA of interest with the probe

Explanation / Answer

Southern Blot -

digest the genomic DNA with a restriction enzyme,separate the DNA fragments by gel electrophoresis,transfer DNA to a positively charged membrace, and detect a fragment of DNA that contain your DNA of interest with the probe

a specific DNA sequence with DNA probe is detected

Northern Blot -

you can detect a amount of certain type of mRNA and can compare that to another sample produced under different experimental conditions

a specific mRNA sequence with a DNA probe is detected

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