Experimental In order to investigate the coffee supplier suspected of fraudulent

Question

Experimental In order to investigate the coffee supplier suspected of fraudulently replacing shipments of high caffeine grounds for the less expensive and lower quality ones, first you will make a 5-point calibration curve of pure caffeine using a biphenyl internal standard ranging from 1-4 mM with respect to caffeine. Once the calibration curve has been generated, you will use it to determine the amount of caffeine in two known coffee samples (one high caffeine, and one the coffee supplier is suspected of using to replace the high caffeine grounds) and a sample taken from the coffee chain.

Explanation / Answer

Answer:

The help for the math to determine the five points for the calibration curve is as below

We need to make a five point calibration curve of pure caffeine using a biphenyl internal standards ranging from 1-4mM with respect to the Caffeine.

The calibration curve to be made for the 1-4 mM range.

The five points can be made for the range 1-4 mM is by adding 0.75 repeatadly.

Thus we need to make the increments of the five points starting form 1 point then keep adding the 0.75 for making another point.

1.75+0.75 = 2.50

2.5+0.75 = 3.25

3.25+0.75 =4.00

The calibration scale for five points will be as 1.00, 1.75, 2.5, 3.25, 4.00

1.00being to show the lowest caffeine

4.00 being to show highest caffeine.

This calibration scale will help us to understand the presence of the caffeine in the samples, by compairng its outcome with the five point scale.  

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