Below is a picture of the lac operon (set the beginning of question 1 for defini

Question

Below is a picture of the lac operon (set the beginning of question 1 for definition of ''operon''). Below you will be asked to predict the outcome of three scenarios involving mutations affecting the lac operon. The effect of each mutation is stated. For each scenario, there will be two questions. ''what products are made?'' and ''when are products made?'' For the question ''What products are made?'' possible answers are: lacZ protein (beta-galactosidase) and/or lacY protein (lactose permease) and/or lacA protein (transacetylase), or none of these proteins. For the question ''When are products made?'' possible answers are: (A) always on (products are always made, regardless of lactose level), always off (products are always made, regardless of lactose level) or (C) regulated (products are made in the presence of lactose, not made in its absence) Scenario #1: the lactose repressor protein can bind to DNA but cannot bind to lactose What products are made? When are products made? Scenario #2: the lactose repressor protein binds to lactose but cannot bind to DNA (iii) What products are Made? When are products made? Scenario #3: the lacA gene is missing What products are made? When are products made?

Explanation / Answer

1--- i) lacZ protein( Beta-Galactosidase) is made

ii) always on.

2---- iii)LacY protein( lactose permease)

iv) always off.

3. v) Trancetylase

vi) regulated

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