In one long paragraph include the following: Use references and provide three pi

Question

In one long paragraph include the following: Use references and provide three pieces of background information on how the quality of water is characterized. Then clearly state the hypothesis and prediction about your water sample. Use one or two sentences to connect your background information with the importance of your hypothesis and your prediction for the experiment. Male s statement about positive and negative controls in the assays. Describe generally how the assays and oxygen titration were done. Describe what Coliscan Easygel is and what it identifies.

Explanation / Answer

QUALITY OF WATER AND DETERMINATION OF ANY CONTAMINATION ;

OXYGEN TITRATION IS DONE BY WRINKLER TEST , TO DETERMINE THE CONCENTRATION OF OXYGEN DISSOLVED . IN THIS ASSAY OXYGEN IS PRECIPIATED WITH MEGNESIUM , SALTS TO FORM A PRECIPITATE WHICH IS THEN ACIDIC HYDROLYSED TO CONVERTED INTO IODINE .SO THE AMOUNT OF OXYGEN IS DIRECTLY RELATED TO IODINE PRESENT WITH THIO SUPHATE INDICATOR .

COLISCAN GEL ;

IT IS USED TO IDENTIFY THE DIFFRENT FORMS OF COLIFORMS WHICH ARE GRAM NEGATIVE AND USES THE LACTOSE AS SUBSTRATE WHICH FORMS THE GAS BUBBLES IN THE PLATE . SO THIS GEL TAKES THE ADVANTAGE OF PRODUCTION OF ENZYME BETA GALACTOSIDASE . THIS GEL CONTAINS THE TWO CHROMOGENIC SUBSTANCES FOR THE GALACTOSIDASE PINK COLOUR AND GALACTOURINASE PURPLE COLOUR PRODUCING TWO DIFFRENT COLOURS IN THE MEDIA . WHEN THERE NO COLOUR THEN THERE ARE NOT COLIFORMS IN THE PLATE

CHARACTERSIZATION OF WATER MOLECULE;

IT DEPENDS ON PHYSICAL , BIOLOGICAL RADIOLOGICAL    CHARECTER OF WATER . THERE WILL BE DIFFRENT INDICATORS FOR THE WATER MOLECULES SAYS THAT IT IS CONTAMINATED , ALKANITY , PH , TASTE AND ODOUR , DISSOLVE METALS , HEAVY METALS ETC , CHEMICAL INDICATORS INCLUDE COD , BOD , TOTAL HARDNESS OF WATER , BIOLOGICAL INDICATORS INCLUDE   PRESENCE OF COLIFORMS , MOLLUSCA , , E.COLI

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