This question is about qPCR. Would anyone be able to explain how to find initial

Question

This question is about qPCR. Would anyone be able to explain how to find initial fluorescence at cycle 0 using the MAK2 method (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0012355)? I understand how the other ones work like the 5PSM method: F0 = Fq/ECq, F0 = initial fluorescence, Fq = fluorescence at Cq, E = efficiency at Cq (fold increase), Cq = quantification cycle (second derivative max). Say for a qPCR run over 30 cycles we get this: "610 618 627 632 628 634 634 628 634 642 637 643 653 666 684 717 787 923 1197 1716 2638 4077 5461 7007 8561 9994 11278 12382 13382 14252". Cq = 20, Fq = 1716, E = 1.433, F0 = 1.287.

Explanation / Answer

Based on the given data,

The mechanistic mak2 model is a recursive fitting method of the qPCR, by using this method; we can calculate initial template fluorescence D0 of any qPCR data. We can measure the initial fluorescence at cycle 0 using the MAK2 method with collaborating with fluorescent spectroscopy and other quantifying methods.

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