Cell Biology Please Need Help With Problem ( Part 6) Please and Thank you in adv

Question

Cell Biology

Please Need Help With Problem ( Part 6)

Please and Thank you in advance!

6. (2.0 points) You are curious about the dynamic instability of microtubules and decide to join a lab that works on microtubule polymerization. The people in the lab help you grow some microtubules in culture using conditions that allow you to watch individual microtubules under a microscope. You can see the microtubules growing and shrinking, as you expect. The professor who runs the lab gets in a new piece of equipment, a very fine laser beam, that can be used to sever microtubules. She is very excited and wants to sever growing microtubules at their middle, using the laser beam. What do you expect would happen to the newly exposed plus ends if you were to grow the microtubules in the presence of an analog of GTP that cannot be hydrolyzed, and you then severed the microtubules in the middle with a laser beam?

Explanation / Answer

DYNAMIC INSTABILITY AND MICROTUBULES;

MICROTUBULE FORM THE CYTOSKELETON OF THE MUSCLES

, IN HUMANS IT IS FORMED WITH THE DIMERIZATION ALPHA AND BETA TUBULIN . DYNAMIC INSTABILITY REFERS TO ARRANGEMENTS OF MICROTUBULES AT THEIR ENDS WHICH USES THE GTP MOLECULE . GTP MOLECULE BINDS TO DIMERS OF TUBULINS IN PLOYMERIZATION STATE . THE GTP BOUND TO ALPHA TUBULIN IS STABLE , GDP MOLECULE BOUND TO BETA TUBULIN CAUSES TO INSATBILITY OF THE MOLECULE . GTP BOUND CAP HELPS THE MICROTUBE FROM DIS ASSEMBLING THER BY PROTECTING IT FROM THE SHRINKAGE . THE STAGE OFROM WHICH MICROTUBLE GROWTH TO SHRIKAGE FORM IS CALLED CATSTROPHIC STAGE , AND CELLS FROM WHICH SHRIKAGE TO GOWTH IS CALLED RESCUE STATE .

USE OF LASER IRRADIATION AND OBSERVATION ;

DURING THE LASER IRRADIATION AN OBSERVATION OF DYNAMIC INSTABILITY CAUSES THERE WILLBE NO GROWTH OR NO SHRINKAGE IS OBSERVED , THE COMPLETELY BOUND GDP TUBULIN WILL NOT RENDER THE CELLS TO UNDERGO CATASTROPHIC STAGE . THE PROOF FOR THAT IS THE CELL GET IOTS ENERGY FROM GTP HYDROLYSIS WHICH OCCURS AT BETA TUBULIN ,WHEN THEY ARE GROWN IN MEDIUM LACK OF GTP THESE CELLS DONOT UNDERGO HYDOLYSIS OF GTP SO THEY ARE NOT UNDERGOING THE DYNAMIC INSTABILITY

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