When disrupting a mouse gene by knockout, why is it desirable to breed mice unti

Question

When disrupting a mouse gene by knockout, why is it desirable to breed mice until offspring homozygous (-/-) for the knockout target gene are obtained?

When disrupting a mouse gene by knockout, why is it desirable to breed mice until offspring homozygous (-/-) for the knockout target gene are obtained?

Please Explain

The knockout gene can be restored in the population. The effect of inheritance cannot be investigated. The knockout gene may cause mutation in the sex cells. The knockout gene cannot be faithfully transmitted at high frequency.

Explanation / Answer

correct answer is

D.The knockout gene cannot be faithfully transmitted at high frequency.

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