For Solution S7, if 30% of BTB are in acidic form, what is the concentration of
ID: 476318 • Letter: F
Question
For Solution S7, if 30% of BTB are in acidic form, what is the concentration of acidic form of BTB in S7? what is teh conecntration of BTB basic form?
Calibration Solution 0.00025M Bromothymol Blue (mL) 0.010 M HCl (mL pimen thrm 1 00 0.010 M NaOH (mL 0.00025M Bromothymol Blue (mL 1.00 0.70 4.30 0.50 4.50 (2) Prepare 5 standard solutions in medium test tubes sing 1 and 5 ml graduated pipettes. Solution 0.000 25M 0.10M 0.10M Bromothymol blue (mL) K2HPO4 (mL) KH2PO4 (mL) 1.00 0.00 4.00 1.00 1.00 3.00 2.00 1.00 2.00 1.00 3.00 S10 1.00 1.00 4.00 0.00 S11Explanation / Answer
In Solution S7; the BTB contains 30% acidic form and when (only) K2HPO4 mixed, the solution S7, pH will be in basic form;
Because pH of the solution of the solution containing only K2HPO4 is approximately 9.7 and BTB pKa is 7.
So, the total BTB will be converted into basic form. The concentration will be :
M1V1 = M2V2
0.00025 M x 1 mL = M2 x 5mL
M2 = 0.00025 M x 1 mL / 5mL = 0.00005 M
The concentration of bromothymol blue after K2HPO4 addition for soultions, S7 is 0.00005 M.
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