81% Tue 43 AM a E Chrome File Edit View History Bookmarks People Window Help Mas

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81% Tue 43 AM a E Chrome File Edit View History Bookmarks People Window Help Mastering Biology: Module Four Lab Work Robbie Bb Lab Materials BIO112 https:// session masteringbiology.co m/myct/itemView?assignmentProblemID-59765803 C https:/ /www.google.co BIO 112 D6B Hel Solve lt: Are You Getting the Fish You Paid For? e screenshot on mac Mod Lab Work Sign in previous l 2 of 14 Web Videos Part D How do you sequence the COI gene from your salmon sample? Now that PCR is complete and you have many copies of your sample's COI gene, you are ready to determine the sequence of nucleotide bases for that gene The sequencing reaction is similar to PCR, but ses a mixture of deoxy-nucleotides (dNTPs, the normal building blocks for DNA) plus fluorescently labeled dideoxy-nucleotides About 34,500,000 res (ddNTPs) that are missing the 3' -OH group and therefore terminate the sequence when they are incorporated into the newly copied DNA. In other words, whenever one of the fluorescent ddNTPs is added, the sequence stop After many cycles of the sequencing reaction, you generate millions of varied length DNA fragments, all ending with a fluorescently labeled nucleotide. Each nucleotide (ddATP, ddTTP Take a screensh ddCTP, or dod GTP) has a different fluorescent color so that the color indicates the final etter" of each sequence fragment. The fragments are then separated by size on an agarose gel. A fluorescence detector measures the intensity and color of the fluorescence of each fragment and prints out sequence 1. Press Commar traces like the ones shown below. Each peak represents the fluorescence detected at that position along the sequence, with the color indicating the base at each position. The 2. Move the cross sequence is read from left to right, 5' to 3 with the shortest fragments at the 5' end to start the scre 3. Drag to select 4. When you've s release your m 5. Find the scree alculus, 10th How to take a La...rds.pdf https://support.appl How to take a emory Clean https://support apple Apr 10, 2015 Take a s Move the crosshair p area. When you've sele Examine the trace for the 10-base sequence of the COI gene shown above. Enter the sequence of nucleotides for this section of the COI gene. Use A for adenine, C for Find the screenshot a cytosine, G for guanine, and T for thymine (e.g., ACGT...). For help reading the trace, see Hint 1. How to screen www.imore.com/scre Submit Hint Give Up Rev Jun 9, 2015 How to t the app and window you ame time. Press the Spacebar, Place e Camera cursor on top e Window u Wal

Explanation / Answer

The sequence is as given below:

5' TACTGATTCT 3'

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