To determine its concentration of iron, a clinician reached a blood serum sample
ID: 500546 • Letter: T
Question
To determine its concentration of iron, a clinician reached a blood serum sample with 10-phenanthroline to give a red-colored complex. the absorbance of the solution was measured to be 0.456. If the molar absorptivity of the red-colored complex is 1.0750 times 10^4 L mol cm and a standard 1.00 cm cuvette was used to make the measurement calculate the concentration of the iron in the serum sample. Present your answer to three significant figures in the format x.xxEx. Include a unit in your answer. If your answer is a compound unit such as g L^-1, present this as g L-1. Note that the correct syntax for this question should have a space between the number and the beginning of the unit as well as a space between the individual parameters in the unit e.g. 4.32E-2 g L-1Explanation / Answer
Since Abosrbance, A= ebC, e= molar absorptivity, C= concentration and b= path length
0.456= 1.075*104 L/molecm * 1cm* C
C= 0.456/(1.075*104)= 4.24*10-5 Moles/L
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