What is the monoisotopic peak? Data: BSA-0001.1B231 May 2007 1122 Cat Training 3
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Question
What is the monoisotopic peak?
Data: BSA-0001.1B231 May 2007 1122 Cat Training 3 Kratos PCAxima CFRplus V2.4-1: Mode reflectron Power: 58.PExt. @2300 bin 102) 238 miMsumF16398 mM Profiles 1-69smooth Akv2-Baseine 80 801 2045.22 2067.24 2132.15 2040 2050 2060 2070 2080 2090 2100 2110 2120 2130 2140 B231 2007.1122 Cai: Training 3 Data BSAr000 Kratos PC Axima CFRpius Mode reflectron Power 58, PExt @2300 bin 102) 24 miMsumme 1678 mM Profilos 1.69 Smooth Aw 2-Basolino 800 2067.24 2132.15 .18 2100 2110 2120 2130 2140 2150 2160 Mass Charge Data BSA-0001,1B231 May 2007 22 Cal Training 31 May 2007 Kratos PC Axima CFRpkrs V2.4.1:Mode reflectron, Power: 58.PExt. @2300 (bin102) 32 mMsume 2228 miM Profiles 1-69 Smooth A 2-Baseine 80 2297.29Explanation / Answer
To understand monoisotopic peak, first you have to understand what is monoisotopic mass,
So, Monoisotopic mass is the sum of masses of the atoms in a molecule using the gfround state , unbound , rest moass of the most abundant isotope for each element. For example , for carbon , the most abundant isotope is c-12 (which is lightest)
But some students got confused with this that always lighter isotop is considered which is totally wrong. LEet us understand with the help of a example that is IRON (Fe) , the most abu8ndant isotope of Fe is Iron-56 which is not the lightest , so relating it with the previous fact , you got understood that we have to consider the most abundant isotope.
So when you look for monoisotropic peak , look for the isotope with the smaller mass , that is , lighter element for smaller peptiudes. BUT if you consider larger peptides, the mono isotropic peak is not the most abundant peak in a spectrum, beecause the probability is higher for peptide to contain atleast 1 naturally occuring isotope.
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