In HPLC, the concentration of a substance can be determined by ... (a) compariso

Question

In HPLC, the concentration of a substance can be determined by ... (a) comparison of the area under the peak produced by the substance with the areas under the peaks produced by standard solutions (b) comparison of the retention time of the substance with that of a standard (c) calculation of the retention time of the substance (d) measurement of the height of the peak produced by the substance In gas chromatography, if two solutes with short retention times co-elute (i.e. are not resolved what is the simplest way to attempt to resolve the peaks? (a) Use a higher column temperature (b) Use a lower column temperature (c) Use a longer column (d) Use a more polar solvent

Explanation / Answer

6)In HPLC,the concentration of substance can be determined by....

Ans:-(a) comparison of area under the peak produced by the substance with the area under the peak produced by standard solution.

17) In gas chromatography if two solutes with short retention time co- elute,(I.E are not resolved what is the simplest way to attempt to resolve the peak?

And:-(b) use lower column temperature.

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