What is the sequence for the following HEXAPEPTIDE? be sure to provide WORK/ANSW

Question

What is the sequence for the following HEXAPEPTIDE? be sure to provide WORK/ANSWER for Points B-F! Observations from Sequencing Experiments amino acid content: Y, R, M, K, G, D B. Reaction with 1-fluoro-2, 4-dinitrobenzene yielded DNP-G. Treatment with chymotrypsin yielded two tripeptides. Treatment with trypsin yielded three dipeptides. Treatment with carboxypeptidase yielded methionine. N-terminal sequencing of one the chymotrypsin-produced tripeptides yield Explanation and Summary of Protein Sequencing Techniques 1-fluoro-2, 4-dinitrobenzene (DNFB) is used to identify the N-terminal amine peptide, and provides the following product: DNP-AA (where AA = the N-

Explanation / Answer

1. DFNB reaction is called sanger method, the reagent reacts with N-terminal amino acid. So it is clear N-y=terminal is G.

2. Chymotrypsin cleaves tyrosine in the chain therefore, as we get two tripeptide, Y must be at place number 3.

3. Trypsin cleaves K and R only, so possiblity is either of K and R is at position 2,4

4. Carboxypeptidase reacts at C-terminal, therefore M is at position 6 (C-terminal)

So far we get,
GKYRDM or GRYKDM

Position of K and R can be confirmed if your part 5 of question is complete

Hope i could help.

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