An arg-strain of E. coli is transformed with a plasmid carrying thewild-type (ar

Question

An arg-strain of E. coli is transformed with a plasmid carrying thewild-type (arg+) gene. The transformed cells are replica-plated totwo minimal medium plates, one supplemented with arginine and onewithout. The transformed cells would grow on
a. only the plate supplemented with arginine b. only the plate lacking arginine c. both plates d. neither plate the plate supplemented with arginine only if a mutationoccurred.
a. only the plate supplemented with arginine b. only the plate lacking arginine c. both plates d. neither plate the plate supplemented with arginine only if a mutationoccurred.

Explanation / Answer

The answer is C. both plates This is because the arg+ gene indicates that the strain ofE.coli can produce arginine on its own. When such a strainis put on a plate, it makes no difference whether the media hasarginine or not. This is becuase it can produce its own supply ofarginine. Therefore, it grows in both the plates

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