Give six different examples of how thin layer chromatography is used in organic

Question

Give six different examples of how thin layer chromatography is used in organic chemistry How do you calculate the R, value for a compound? Increasing the solvent polarity will have what effect on the Ry value? Decreasing the solvent polarity will have what effect on the Ry value? What steps are needed to analyze the components of an analgesic tablet by TLC? Write a brief procedure that you will follow. 6.) What steps are needed to purify the components of an analgesic tablet? Write a brief procedure that you will follow.

Explanation / Answer

Answer for question 1:

Uses of thin layer chromatography in organic chemistry:

i. Qualitative analysis of components in a sample mixture

ii. Quantitative analysis of components in a sample mixture

iii. Visualization of components of a reaction mixture

iv. Monitoring a reaction from beginning to end

v. Monitoring completion of a starting material in a reaction

vi. Monitoring formation of impurities in a reaction

Answer for question 2:

Rf value is a unique value for a given compound in a given single solvent or a mixture of solvents. It can be measured by using the following formula:

Rf value = Distance moved by the given sample on a TLC plate/ Distance move by the given solvent on a TLC plate

Answer for question 3:

Increasing the solvent polarity will increase the Rf value of a given compound.

Answer for question 4:

Decreasing the solvent polarity will decrease the Rf value of a given compound.

Answer for question 5:

Steps involved in the analysis of an analgesic tablet by TLC:

1. Crush the given analgesic tablet to a fine powder

2. Extract the analgesic sample mixture from the powder into a suitable solvent

3. Prepare a TLC plate by spotting the sample mixture along with standards in the mixture

4. Develop the TLC plate in a solvent mixture

5. Visualize the components of sample mixture under UV light or using iodine chamber or by staining

6. Calculate the Rf values of components of sample and compare with that of the standard values

Answer for question 6:

Steps involved in the purification of components of an analgesic tablet:

1. Crush the given analgesic tablet to a fine powder

2. Extract the analgesic sample mixture from the powder into a suitable solvent

3. Analyse the components of given analgesic tablet by TLC as shown above in the question 5

4. Decide method of purification of the given sample, i.e. by HPLC or Silica gel column chromatography

5. Separate the components of given analgesic tablet by chosen method of purification

6. Quantify the individual components of the given analgesic tablet

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