Assume that CyclinD and CDK4 bind. A Gal4-DNA Binding Domain (DBD) is fused to c

Question

Assume that CyclinD and CDK4 bind. A Gal4-DNA Binding Domain (DBD) is fused to cyclinD. The Gal4-Activation Domain (AD) is fused with CDK4. If placed into cells containing the LacZ gene and the Gal4 Upstream Activating Sequence, which of the following is true?                            

                                CDK4 and CyclinD bind causing the AD to bind to the DBD which associates with the UAS and induces LacZ transcription

                                CDK4 and CyclinD bind causing the AD to dissociate from the DBD which represses the UAS and inhibits LacZ transcription

                                LacZ gene transcription is inhibited when CyclinD and CDK4 bind to the UAS.

Lac Z binds to and activates transcription of CDK4 DNA and Cyclin D

                                CDK4 and CyclinD bind causing the AD to bind to the DBD which associates with the UAS and induces LacZ transcription

                                CDK4 and CyclinD bind causing the AD to dissociate from the DBD which represses the UAS and inhibits LacZ transcription

                                LacZ gene transcription is inhibited when CyclinD and CDK4 bind to the UAS.

Lac Z binds to and activates transcription of CDK4 DNA and Cyclin D

Explanation / Answer

Based on the yeast two hybrid assays, the CyclinD and CDK4 interact with each other and express the LacZ gene.

Thus, the correct option is (A): CDK4 and CyclinD bind causing the AD to bind to the DBD which associates with the UAS and induces LacZ transcription.

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.