Use the following formulas to solve the problem Nt=N0 x2^n k=n/?t k=1/g g=1/k 1.
ID: 55491 • Letter: U
Question
Use the following formulas to solve the problem
Nt=N0 x2^n k=n/?t k=1/g g=1/k
1. You conduct a growth curve experiment to determine the growth rate of a newly isolated bacterium. In this experiment, you use spectrophotometry to monitor the increase in bacterial cell growth. The data is shown in the table below.
Use the information in the table to answer the following questions.
(a) What is the growth rate of this bacterium?
(b) What is the doubling time for this bacterium in minutes?
OD600 0.05 0.08 0.13 0.20 0.33 Time (min) 20 40 60 80Explanation / Answer
a) growth rate u=ln 2/ td = 0.693/ 30 mins = 0.0231 min -1
b) According to the data given above, the doubling time for this bacterium is 30mins.
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