Given the dilution series outlined below: A. Give the dilution used for each ste
ID: 55793 • Letter: G
Question
Given the dilution series outlined below:
A. Give the dilution used for each step (A-E).
B. (i) What is the total dilution at each step (A-E)?
(ii) What is the resulting dilution found on each plate (F,G,H)?
C. If the original broth culture contains 3 x 10^8 cells ml^-1,
(i) How many bacteria ml^-1 are in each tube (A-E)?
(ii) How many colonies can be found on each plate (F,G,H)?
(iii) Which are the most reliable colony counts, those from plate F, G, or H?
D. If tube C contains 7 x 10^4 bacteria ml^-1, how many bacteria ml^-1 were in the original culture and in each of the other tubes? How many colonies can be found on each plate?
0.1m .0m 0.1m 1.0 0.5ml 1.0m 1.0ml 0.1ml 9.9ml 9.9m 9.0m 4.5m 1.0ml 8Explanation / Answer
Q A.
A---->10-2 times dilution from the stock
B------>10-4 times dilution from the stock
C ------> 10-5 times dilution from the stock
D-------> 10-6 times dilution from the stock
E -----> 0.5* 10-6 times dilution from the stock
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