Cells from two patients, those with a normal (wild type) GlcNac transferase I an

Question

Cells from two patients, those with a normal (wild type) GlcNac transferase I and those without GlcNac transferase I (mutant). Which of the following is/are true?

Incubation of the trans-Golgi of wild type with mutant Golgi causes the addition of GlcNac to an N-linked glycoprotein

Incubation of the trans-Golgi of mutant with wild type Golgi causes the addition of GlcNac to an N-linked glycoprotein

Incubation of the trans-Golgi of wild type with mutant Golgi causes the addition of GlcNac to an N-linked glycoprotein

Incubation of the trans-Golgi of mutant with wild type Golgi causes the addition of GlcNac to an N-linked glycoprotein

Explanation / Answer

Incubation of the trans-Golgi of mutant with wild type Golgi causes the addition of GlcNac to an N-linked glycoprotein is true because the wild type is already capable of the addition of GlcNac to an N-linked glycoprotein and hence, when mutant which is not capable of this reaction is incubated it may gain the activity. Option 2 is correct.

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