Find and mark the region in the partial Hemagglutinin (HA) gene sequence below t

Question

Find and mark the region in the partial Hemagglutinin (HA) gene sequence below that codes for the peptide:

Met-Lys-Thr-Ile-Ile-Ala-Leu-Ser-Tyr-Ile…

5’GAATGTAGCTCAAAGCAATGATAGTCTTCATGGTTA 3’ 3’CTTACATCGAGTTTCGTTACTATCAGAAGTACCAAT 5’

Practice Problems

1. Find the stretch of DNA in the following sequence that codes for the peptide:

Q E G E I V V L V Q N

5’ACAAAAAATGATGAAGGCAATCATTCCTCATAAGATAATAAAAAGTGTATTGAGAGTGA 3’ 3’TGTTTTTTACTACTTCCGTTAGTAAGGAGTATTCTATTATTTTTCACATAACTCTCACT 5’

5’GTGAGCATGAAAAAGATTTAGTATTTAGCAGTGCGGATATGATCCAAGAGGGTGAAATA 3’ 3’CACTCGTACTTTTTCTAAATCATAAATCGTCACGCCTATACTAGGTTCTCCCACTTTAT 5’

5’GTCGTTCTCGTTCAGAATCTTTTGCAGCATAAGTAGTATGTCGATATACTTATCGTTGA 3’ 3’CAGCAAGAGCAAGTCTTAGAAAACGTCGTATTCATCATACTGCTATATGAATAGCAACT 5’

2. The following gene fragment contains the coding sequence for the beginning of a 394 amino acid. What are the likely first five amino acids for the protein?

5’AAGCTTCAGCGCGAACGACCAACTACCCCGATCATCAGTTATCCTTAAGGTCTCTTTTG 3’ 3’TTCGAAGTCGCGCTTGCTGGTTGATGGGGCTAGTAGTCAATAGGAATTCCAGAGAAAAC 5’

5’TGTGGTGCGTTCCGGTATGGGGGGGGCTGCCGCCAGGTTGGGGGCCGTGATTTTGTTTG 3’ 3’ACACCACGCAAGGCCATACCCCCCCCGACGGCGGTCCAACCCCCGGCACTAAAACAAAC 5’

5’TCGTCATAGTGGGCCTCCAGGGGGTCCGCGGCAAATATGCCTTGACGGACGCCTCTCTC 3’ 3’AGCAGTATCACCCGGAGGTGCCCCAGGCGCCGTTTATACGGAACTGCCTGCGGAGAGAG 5’

3. The DNA sequence below contains the entire coding region for a gene. Below that is the amino acid sequence of the protein coded for by the gene. A cell suffers a mutation that results in an altered protein being produced (shown below). Describe the location and type of mutation that has occurred.

DNA sequence:

5’AACAGCATAAACACAAAATCCATCAAAAATGTCGATGAAATATCTGATGTTGTTGTTCG 3’ 3’TTGTCGTATTTGTGTTTTAGGTAGTTTTTACAGCTACTTTATAGACTACAACAACAAGC 5’

5’CTGCTATGATAATTAGATCATTCGCCAATAGTGGTAACGCTATCGAAACGACATTGTCA 3’ 3’GACGATACTATTAATCTAGTAAGCGGTTATCACCATTGCGATAGCTTTGCTGTAACAGT 5’

5’AGAATTACAAACACTACAACAGATATTCCAGCTATCTGATTATGCGGTCCAGAGGGAAA 3’ 3’TCTTAATGTTTGTGATGTTGTCTATAAGGTCGATAGACTAATACGCCAGGTCTCCCTTT 5’

Original Protein Sequence: M L L F A A M I I R S F A N S G N A I E T T L S R I T N T T T D I P A I

Mutated Protein Sequence: M L L F A A M I I R S F A N S G N A N R N D I V K N Y K H Y N R Y S S Y L I M R S R G K

Explanation / Answer

5’GAATGTAGCTCAAAGCAATGATAGTCTTCATGGTTA 3’

3’CTTACATCGAGTTTCGTTACTATCAGAAGTACCAAT 5’

The sequence in the bold code for the peptide sequence in the HA partial sequence.

1.

5'ACAAAAAATGATGAAGGCAATCATTCCTCATAAGATAATAAAAAGTGTATTGAGAGTGA 3’ 3’TGTTTTTTACTACTTCCGTTAGTAAGGAGTATTCTATTATTTTTCACATAACTCTCACT 5’

5’GTGAGCATGAAAAAGATTTAGTATTTAGCAGTGCGGATATGATCCAAGAGGGTGAAATA 3’ 3’CACTCGTACTTTTTCTAAATCATAAATCGTCACGCCTATACTAGGTTCTCCCACTTTAT 5’

5’GTCGTTCTCGTTCAGAATCTTTTGCAGCATAAGTAGTATGTCGATATACTTATCGTTGA 3’ 3’CAGCAAGAGCAAGTCTTAGAAAACGTCGTATTCATCATACTGCTATATGAATAGCAACT 5’

The sequence highlighted in bold code for the following peptide QEGEIVVLVQN

2. The likely first five aminoacids of the protein is MGGAAAR...

3.

5’AACAGCATAAACACAAAATCCATCAAAAATGTCGATGAAATATCTGATGTTGTTGTTCG 3’ 3’TTGTCGTATTTGTGTTTTAGGTAGTTTTTACAGCTACTTTATAGACTACAACAACAAGC 5’

5’CTGCTATGATAATTAGATCATTCGCCAATAGTGGTAACGCTATCGAAACGACATTGTCA 3’ 3’GACGATACTATTAATCTAGTAAGCGGTTATCACCATTGCGATAGCTTTGCTGTAACAGT 5’

5’AGAATTACAAACACTACAACAGATATTCCAGCTATCTGATTATGCGGTCCAGAGGGAAA 3’ 3’TCTTAATGTTTGTGATGTTGTCTATAAGGTCGATAGACTAATACGCCAGGTCTCCCTTT 5’

At the position of nucleotides underlined, nucleotide A was added by the mutation which changed the total protein sequence. This type of mutation is called insertion mutation.

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