An unusual new species of bacteria is isolated from terrapin gut. It lacks the m

Question

An unusual new species of bacteria is isolated from terrapin gut. It lacks the mismatch repair system found in essentially all other organisms. Which of the following do you expect to be true about this bacteria? It will have a lower spontaneous mutation rate than E. coli. It will be unusually sensitive to the formation of double strand breaks in its DNA. It will not be able to incorporate 5-Bu into its DNA. It will have a relatively high level of SNP polymorphisms. It will not score touchdowns. You decide to carryout undergraduate research in a cockroach genetic lab. You isolate a spontaneous mutation that results in a lack of pigmentation in the eye. You guess that this mutation might be in the cockroach homolog of the fly while gene. You examine the recent cockroach genome sequence and find there is a single homolog of the fly while gene. This gene lacks any obvious introns and the ORE spans 2 kb. You use PCR to obtain DNA that you can use as a probe for the ORF of the gene. You isolate genomic DNA from wild type and mutant cockroaches, cut them with EcoR1, fractionate the DNA by gel electrophoresis, do a Southern blot and then hybridize the blot with labeled cockroach while gene. You get the following result: You interpretation of this is:

Explanation / Answer

11. It will be unusually sensitive to the formation of double strand breaks in its DNA

12. The mutation is likely to do a frameshift mutation that inactivated the white gene of cockroach

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