The following reagents are often used in protein chemistry. Match the reagent wi

Question

The following reagents are often used in protein chemistry. Match the reagent with the purpose for which it is best suited. Some answers may be used more than once or not at all; more than one reagent may be suitable for a given purpose. CNBr (cyanogen bromide) chymotrypsin trypsin Edman reagent (phenylisothiocyanate) Sanger reagent (FDNB) reducing agent (beta Mercaptoethanol) _hydrolysis of peptide bonds on the carboxyl side of Lys and Arg _cleavage of peptide bonds on the carboxyl side of Met _breakage of disulfide (-S-S-) bonds _determination of the amino acid sequence of a peptide _determining the amino-terminal amino acid in a polypeptide

Explanation / Answer

a. Cyanogen bromine - cleavage of peptide bonds on the carboxyl side of Met

b. Edman degradation - determining the amino-terminal amino acid in a polypeptide

c. Sanger reagent - determination of the amino acid sequence of a peptide

d. Reducing agent - breakage of disulfide (-S - S-) bonds

e. Chymotrypsin:

f. Trypsin - hydrolysis of peptide bonds on the carboxyl side of Lys and Arg

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.