This is for cell biology and the professor said that only thing we need are the

Question

This is for cell biology and the professor said that only thing we need are the websites listed below. Here's the questions

1. What is the protein sequence predicted for the murine cDNA sequence below?

5' - GAGCGCTTCA GCAGGGAAGA TTCCCTTCCC CCCGCTTCAG GCTGCTGAGC ACTGAGCGGC      GCTCAGAATG GAAGCCATCG CCAAATATGA CTTCAAAGCT ACTGCTGACG ATGAGCTGAG      CTTCAAGAGG GGGGACATCC TTAAGGTTTT GAATGAAGAG TGTGATCAGA ACTGGTATAA      GGCAGAACTC AATGGGAAAG ATGGCTTCAT CCCCAAGAAT TACATAGAAA TGAAACCACA      TCCGTGGTTT TTTGGCAAAA TCCCCAGAGC AAAGGCAGAA GAAATGCTTA GCAAACAGCG      GCATGACGGG GCCTTCCTGA TCCGAGAGAG CGAGAGCGCT CCTGGGGATT TCTCCCTGTC      AGTCAAGTTT GGAAATGATG TACAGCACTT CAAGGTGCTT CGTGACGGAG CCGGGAAGTA      CTTCCTGTGG GTGGTGAAGT TTAATTCTTT GAATGAGCTG GTAGATTACC ACAGATCAAC      ATCCGTGTCC AGGAACCAGC AGATATTCCT CCGGGACATA GAACAGGTGC CACAGCAGCC      AACGTACGTC CAGGCACTCT TTGACTTTGA CCCCCAGGAG GATGGTGAGC TGGGCTTTCG      CCGAGGAGAC TTCATTCATG TCATGGATAA CTCAGATCCC AATTGGTGGA AAGGGGCCTG      CCATGGGCAG ACCGGCATGT TTCCCCGCAA TTATGTCACC CCAGTGAACC GGAACGTCTA      AGAAGCAAAA GAGATTATTT AAAGAAAGTG AAAAGTTGAG ACCGTTCACA AGAATTACAC      CCACACGCTG CCTGTCACAG CCTGTGAGGG AACGCAGAAC ACCTGGCTGG GTCCCACGGG    AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA - 3'

2. What are the molecular weight and isoelectric point(pI) values for the hypothetical protein?

3. What is the domain structure of the hypothetical protein?(create a screenshot and paste into your answer sheet)

Use the following websites for these questions:

http://web.expasy.org/translate/ <-----this is to translate sequence

http://web.expasy.org/compute_pi/ <-------this is for question 2

http://smart.embl-heidelberg.de/ <--------this is for 3

I have no idea how to use these sites. Please help?

Explanation / Answer

According to the website given "http://web.expasy.org/translate/" , all six reading frames will give a protein sequence.

Well, according to me, the best sequence is :-

5'3' Frame 2
S A S A G K I P F P P L Q A A E H Stop A A L R Met E A I A K Y D F K A T A D D E L S F K R G D I L K V L N E E C D Q N W Y K A E L N G K D G F I P K N Y I E Met K P H P W F F G K I P R A K A E E Met L S K Q R H D G A F L I R E S E S A P G D F S L S V K F G N D V Q H F K V L R D G A G K Y F L W V V K F N S L N E L V D Y H R S T S V S R N Q Q I F L R D I E Q V P Q Q P T Y V Q A L F D F D P Q E D G E L G F R R G D F I H V Met D N S D P N W W K G A C H G Q T G Met F P R N Y V T P V N R N V Stop E A K E I I Stop R KStop K V E T V H K N Y T H T L P V T A C E G T Q N T W L G P T G K K K K K K K K K K K K K K K K K K K

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