The cells we are transforming lack LacZ in the bacterial genome. What do you exp

Question

The cells we are transforming lack LacZ in the bacterial genome. What do you expect would happen if we transformed LacZ+ cells with your plasmid? Why would it be a problem?

In this experiment, each plasmid will contain the following genes.

1) A gene, AmpR, that encodes a gene that confers antibiotic resistance
2) A gene, LacZ, that codes for beta-galactosidase, which is an enzyme that can break down lactose into its constituent monosaccharrides, glucose and galactose

3) An origin of replication, so that the plasmid can be replicated.
4) A multiple cloning site with a number of sites recognized by a variety of restriction enzymes.

Explanation / Answer

When we clone bacteria with a plasmid that is LacZ and is ampicillin resistant, the ampicillin resistant genes is inserted in the LacZ gene of recombinant bacteria. So, beta galactosidase will not be produced by the recombinant cells.

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