Order the steps that would be used in a laboratory to engineer a bacterium that

Question

Order the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor VIII. Not all steps will be placed.

Isolate the human gene that produces factor VIII

-Generate cDNA of the factor VIII gene using reverse transcriptase

- Transform the vector into E. coli bacterium

-The factor VIII protein is isoloated from human tissues

-Insert the factor VIII cDNA into a bacterial vector near a promoter site

-Human DNA is introduced into the bacterial cell using direct injection by a small needle

-Isolate the mRNA of the factor VIII gene

E.coli expresses factor VIII

Explanation / Answer

Ans : Order the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor VIII.

Steps order :

-- Isolate the human gene that produces factor VIII

--- Isolate the mRNA of the factor VIII gene .

--- Generate cDNA of the factor VIII gene using reverse transcriptase.

---- Insert the factor VIII cDNA into a bacterial vector near a promoter site.

--- Transform the vector into E. coli bacterium.

--- E.coli expresses factor VIII.

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