Answer all of the following questions concerning next-generation sequencing plea

Question

Answer all of the following questions concerning next-generation sequencing please!

What are the two methods of next-generation sequencing we have discussed? [I believe the two types of sequencing are Pyrosequencing and Reversible Terminator Sequencing]

Describe one similarity between the two methods.

Describe one difference between the two methods.

These technologies can be used for much more than simple genome sequencing. Describe one use of next-generation sequencing beyond sequencing the genome of an organism.

Explanation / Answer

The sequencing of cDNA rather than genomic DNA focuses analysis on the transcribed portion of the genome. This focus reduces the size of the sequencing target space, which can be viewed as desirable given the fact that, even with next-generation sequencers, sequencing an entire vertebrate genome is still an expensive undertaking. Transcriptome sequencing has been used for applications ranging from gene expression profiling, genome annotation, and rearrangement detection to noncoding RNA discovery and quantification. A unique feature of high-throughput transcriptome sequencing studies is the versatility of the data, which can simultaneously be analyzed to provide insight into the level of gene expression, the structure of genomic loci, and sequence variation present at loci (e.g., SNPs). To date, the 454 technology has dominated next-generation applications in transcriptomics; but at least one recent paper describes the use of the Illumina sequencer for profiling microRNAs

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