Restriction enzymes are named according to the species in which they are found.

Question

Restriction enzymes are named according to the species in which they are found. The first three letters are italicized because they indicate the genus and species names. Because a species may produce more than one restriction enzyme, the enzymes are designated I, II, III, and so on, to indicate the order in which they were The recognition sites for several different restriction enzymes are shown in Table 20.3. After these restriction enzymes have cleaved the DNA, four of them produce sticky ends that can hydrogen bond with complementary sticky ends. The efficiency of sticky ends binding together depends on the number of hydrogen bonds; more hydrogen bonds makes the ends "stickier" and more likely to stay attached. Rank these five restriction enzymes in Table 20.3 (from best to worst) with regard to the efficiency of their sticky ends binding to each other.

Explanation / Answer

Best to worse.

BamHI = Sau3AI = PstI > EcoRI > NaeI i.e. NaeI is least sticky producing blunt ends, EcoRI is better having 8 hydrogen bonds at sticky end where as BamHI, Sau3AI, PstI have 10 each.

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