Can anyone tell me what a reasonable mutation rate would be in EMS treated E.col

Question

Can anyone tell me what a reasonable mutation rate would be in EMS treated E.coli?
I need a reasonable rate for a 10^-5 and 10^-6 dilutions of EMS treated E.coli.

Explanation / Answer

Im not sure how you would calculate this exactly, but I know that in order to calculate mutation rates you have to determine the mutations frequency in the population. Since you are using EMS which causes AT -> GC and GC -> AT transversion mutations; the calculation for mutation rates is something like this... Mutation rates are probability of the number of mutations per nucleotide pair per generation the forward mutation rate AT -> GC is u the reverse mutation rate GC -> AT is v The frequency of AT is p the frequency of GC is q (these frequencies can be found and will be specific to the strain) the equilibrium is q = (u)/(u+v) p = 1-q since p and q are known values that can be found you can find u and v the total mutation rate for this mutagen would be the sum of all the probabilities so u +v here are also some articals I found on the subject and the mutagenic effects of EMS: http://jb.asm.org/cgi/reprint/135/2/466.pdf http://www.ncbi.nlm.nih.gov/pubmed/6390190?dopt=AbstractPlus&holding=f1000,f1000m,isrctn http://www.ncbi.nlm.nih.gov/pmc/articles/PMC304203/?page=1

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