1.If you want to build up a cDNA library, which of the following is NOT necessar
ID: 69311 • Letter: 1
Question
1.If you want to build up a cDNA library, which of the following is NOT necessary?
Ligase
Endogenous restriction enzyme
RNA polymerase
Reverse transcription DNA polymerase
2. What will be produced when digest a DNA fragment which contains restriction site with endogenuous enzymes?
Neither a nor b
Sticky ends
Either a or b
Blunt ends
3. Given the following plasmid, what are the sizes of the fragments you would expect from a NotI/EcoRI double digest?
1.5 kb, 3 kb, 5.5 kb
2 kb, 3 kb, 5 kb
1 kb, 1.5 kb, 2 kb, 2.5 kb, 3 kb
2.5 kb, 2.5 kb, 5 kb
4. About the restriction-modification systerms, which of the following statement is CORRECT?
Bacterial cells can modify the restriction sequence by addition of methyl groups and thus protect the bacteria's own DNA from being digested by endogenous restriction enzymes
Bacterial cells can modify the restriction sequence by addition of methyl groups and thus promote the bacteria's own DNA to be digested by endogenous restriction enzymes
Bacterial cells can modify the restriction sequence by addition of acetyl groups and thus promote the bacteria's own DNA to be digested by endogenous restriction enzymes
Bacterial cells can modify the restriction sequence by addition of acetyl groups and thus protect the bacteria's own DNA from being digested by endogenous restriction enzymes
a.Ligase
b.Endogenous restriction enzyme
c.RNA polymerase
d.Reverse transcription DNA polymerase
Explanation / Answer
1. a. Ligase
2. Sticky ends
3.
4. Bacterial cells can modify the restriction sequence by addition of acetyl groups and thus protect the bacteria's own DNA from being digested by endogenous restriction enzymes
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