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saplinglearning.com 0 Home - NetSuite. [5) Sigma Nu (Ful Menu l Jimmy Jo httos:/cdn.jimmy Files Sapling Learning University of Sou... www.saplinglearn. ATP hycrolysis m.. Chemistry questi Sapling Learning macmilan learring Jump to... Max Scott Sapling LearningUniversity of Southern Mississippi, Hattiesburg-CHE 420- Fall17 KESSL Activities and Due Dates FINAL EXAM My Assignment Resources Score 12/7/2017 04:00 PM 29.5/100 12/7/2017 02:00 PM Time Remaining: 1 H 45 M Gradebook Assignment Information Pnnt Calculatord Periodic Table - 95 Available From: 127/2017 01:30 PM Due Date Points Possible: 100 Grade Category: Exam 4 Description Policies: 10 90 12/7/2017 04:00 PM Sapling Learning The Lineweaver-Burk plot shown below, which plots the r reciprocal of the substrate concentration S], is a typically used to determine the maximum rate, V, and the Michaelis constant, Km hich can be gleaned from the intercepts and slope. Identify each intercept and the slope in terms of the constants Vinax and Km. of the reaction rate(1)versus the representation of enzyme kinetics. This plot is 1.00 12 13 Comp Final Lineweaver-Burk Plot You can check your answers. You can view solutions after the due date. You have three attempts per question. You lose 5% of the points available to each answer in your question for each incorrect attempt at that 14 15 Slope DeTextbook 18 19100 o Help With This Topic 20 [S O Web Help& Videos 21 O Technical Support and Bug Reports 90 23 90 PrevousCheck Answer NtExt Explanation 24 1 100 2011 2017 Sapling Learning, Inc about us careersprivacy poicy terms ofuse contact us help

Explanation / Answer

Recall that this plot is used for Michaelis –Menten equation. It helps to calculate the constants much easier (via graph)

From Michaelis–Menten equation.

V = Vmax*[S] / (Km + [S])

Where

Vmax = max rate velocity (M/s)

[S] = substrate concentration (M)

Km = Michaelis–Menten constant [M]

V = reaction rate [M/s]

Now… mathematical manipulation

Inverse:

1 / [ V = Vmax*[S] / (Km + [S]) ]

1/V = (Km + [S]) / (Vmax*[S])

Separate species

1/V = (Km) / (Vmax*[S])  + [S] / (Vmax*[S])

1/V = Km / Vmax*[S]  + 1/ Vmax

Now, note that:

1/V = Km / Vmax*[S]  + 1/ Vmax

1/V = y-axis

Km/Vmax = slope

1/[S] = x-axis

1/Vmax = y-intercept

-1/Km = x-intecept ( when y = 0 )

Now… Plot the your DATA:

x-axis = 1/[S]

y-axis = 1/V

Using a spreadsheet program, get the equation

Slope = Km/Vmax  

y-intercept = 1/Vmax  

x-intercept = -1/Km

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