B-gal experiment. Please help?? 1. Why didnt we use lactose as the substrate in
ID: 70145 • Letter: B
Question
B-gal experiment. Please help??1. Why didnt we use lactose as the substrate in la?
2.What was the advantage of using ONPG?
3.What is the name of the human homog of B-galactosidase?
B-gal experiment. Please help??
1. Why didnt we use lactose as the substrate in la?
2.What was the advantage of using ONPG?
3.What is the name of the human homog of B-galactosidase?
1. Why didnt we use lactose as the substrate in la? 1. Why didnt we use lactose as the substrate in la?
2.What was the advantage of using ONPG?
3.What is the name of the human homog of B-galactosidase?
Explanation / Answer
2. ONPG allows the colorimetric and spectrophotometric detection of beta-galactosidase activity. This chemical is normally color less, but when beta-galactosidase is present, it splits into galactose and ortho-nitrophenol, which gives a yellow color.
3. Human -glucuronidase is homologous to the Escherichia coli enzyme -galactosidase.
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