B-The difference between clone by clone sequencing and shotgun sequencing is 1.
ID: 9900 • Letter: B
Question
B-The difference between clone by clone sequencing and shotgun sequencing is
1. clone by clone sequencing is faster and more accurate than shotgun sequencing.
2. Bioinformatics can be used for clone by clone sequencing, but not for shotgun cloning.
3. clone by clone sequencing uses small randomly cut pieces of DNA, and uses a computer to put together the overlaps.
4. clone by clone sequencing aligns large contigs of DNA.
C-Haemophilus influenzae was the first organism to have its entire genome sequenced. Which approach was used to sequence its genome?
1. reverse transcription
2. shotgun DNA sequencing
3. mapping with transposition
4. clone-by clone sequencing
D-In addition to microscope slides, ____ may be used for microarrays.
1. microvials
2. transistors
3. silicone chips
4. gel sheets
5. microfiches
1. mass spectrophotometry
2. gel electrophoresis
3. centrifugation
4. sedimentation
5. thin-layer chromatography
Explanation / Answer
A-In the construction of physical maps, the technique of _____ is used to separate restriction digested or PCR generated DNA fragments.
1. mass spectrophotometry
2. gel electrophoresis
3. centrifugation
4. sedimentation
5. thin-layer chromatography
B-The difference between clone by clone sequencing and shotgun sequencing is
1. clone by clone sequencing is faster and more accurate than shotgun sequencing.
2. Bioinformatics can be used for clone by clone sequencing, but not for shotgun cloning.
3. clone by clone sequencing uses small randomly cut pieces of DNA, and uses a computer to put together the overlaps.
4. clone by clone sequencing aligns large contigs of DNA.
C-Haemophilus influenzae was the first organism to have its entire genome sequenced. Which approach was used to sequence its genome?
1. reverse transcription
2. shotgun DNA sequencing
3. mapping with transposition
4. clone-by clone sequencing
D-In addition to microscope slides, ____ may be used for microarrays.
1. microvials
2. transistors
3. silicone chips
4. gel sheets
5. microfiches
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