c) What is happening in lane 2? You have treated a plasmid with 3 different rest

Question

c) What is happening in lane 2?

You have treated a plasmid with 3 different restriction enzymes and run the result in DNA fragments on a DNA agarose gel. Unfortunately, you forgot to keep good records, and the only things you can remember is that your standards (a set of well characterized DNA "markers" with known sizes, sizes are shown on right in kilobase pairs) are in Lane 5 and your uncut control is in Lane 1, and that you loaded roughly the same amount of total DNA in your sample lanes Given no other information approximately how big do you think your original plasmid is How many times did the enzyme(s) used in lane 2 digest the plasmid How many times did the enzyme(s) used in lane 4 digest the plasmid Explain. What is happening in lane 2

Explanation / Answer

a) Approximately the original plasmid has the size of about 18 kbps.

b) The enzymes digested the sample in lane 2 for two times.

The enzymes digested the sample in lane 4 for two times.

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