You are trying to determine whether infection of neurons with coxsackie virus ca

Question

You are trying to determine whether infection of neurons with coxsackie virus causes apoptosis. You decide to look at cytochrome c and DNA fragmentation in your cells. Which of the following results would indicate that your cells are undergoing apoptosis?

cytochrome c will be present throughout the whole cell and a ladder of DNA fragments will be seen on the DNA gel

cytochrome c will be present throughout the whole cell and one band will be seen on the DNA gel

cytochrome c will be present in the mitochondria matrix and a ladder of DNA fragments will be seen on the DNA gel

cytochrome c will be present in the mitochondria intermembrane space and a ladder of DNA fragments will be seen on the DNA gel

cytochrome c will be present throughout the whole cell and a ladder of DNA fragments will be seen on the DNA gel

cytochrome c will be present throughout the whole cell and one band will be seen on the DNA gel

cytochrome c will be present in the mitochondria matrix and a ladder of DNA fragments will be seen on the DNA gel

cytochrome c will be present in the mitochondria intermembrane space and a ladder of DNA fragments will be seen on the DNA gel

Explanation / Answer

Option A is a correct answer, cytochrome C release from mitochondria to the cytoplasm (throughout the cell) during apoptosis and DNA fragmentation is also a late apoptotic event.

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