Two funding applications, A and B, propose utilization of Para magnetic Relaxati

Question

Two funding applications, A and B, propose utilization of Para magnetic Relaxation Enhancement (PRE)to study the state (extended vs. compacted) of inter-domain linkers in two proteins: 120-residue linker (proposal A) and 10-residue linker (proposal B). The domains on the either ends on both linkers are the same in size (50-residue) and all confirmed to be folded. To distinguish the extended vs. compacted states of the linkers, the paramagnetic label (same one in both cases) is to be placed specifically in the middle of the linkers sequence. Circle what application(s) you would suggest to fund: For the 110-residue protein in proposal B (above), sketch the possible graph (assume no pro lines): "backbone amide proton ^1H_N chemical shift values (in ppm) vs. residue number" if the linker is extended.

Explanation / Answer

To distinguish the states of the linkers, the paramagnetic labels areto be placed in middle of both of the linker sequences. The labels are placed one at a time, on the surface of both NTD (N-terminal domain) and CTD (c-terminal domain). Therefore, the correct answer is "both A and B."

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