In a protein determination, the color of dye changes to blue when it binds to pr

Question

In a protein determination, the color of dye changes to blue when it binds to protein, and the absorbance of light is measured. The data below were taken to calibrate the instrument. An unknown protein sample gave an absorbance of 0.867 ( corresponding to the average of three replicate analyses of the unknown sample). Calculate the mass (micrograms) of protein in the unknown. Calculate the absolute uncertainty for the answer.

Data:
Protein (micrograms): 0.00, 9.37, 18.73, 28.08, 37.44
Absorbance: 0.490, 0.720, 0.850, 1.110, 1.250

Explanation / Answer

plotting the graph will gives you the value reading from the graph unknown mass=20 micrograms

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