When the Vmax and the Km of an enzyme are not known, it is essential to determin

Question

When the Vmax and the Km of an enzyme are not known, it is essential to determine the enzyme's initial velocity (Vo) at different [S]. To determine the initial velocity, one would have to:
Select one:
a. Use the Michaelis Menten equation
b. Measure the amount of product formed after the reaction has reached equilibrium
c. Measure the change of [P] vs time in the initial, linear phase of the experiment
d. Find the substrate concentration that gives half the Vmax
e. Search the internet for this enzyme's Vo values


Which of the following statements about a plot of Vo vs. [S] for an enzyme that follows Michaelis-Menten kinetics is FALSE?
Select one:
a. Km is the [S] at which Vo = 1/2 Vmax.
b. The shape of the curve is a hyperbola.
c. The y-axis is a rate term with units of milimoles/min.
d. As [S] increases, the initial velocity of reaction, Vo, always increases.



For the reaction A ? B, ?G = -60 kJ/mol. The reaction is started with 10 mmol of A. no B is initially present. After 24 hours, analysis reveals the presence of 0.2 mmol of B, 9.8 mmol of A. Which is the most likely explanation?
Select one:
a. A and B have reached equilibrium concentrations.
b. Formation of B is thermodynamically unfavorable.
c. The result described is impossible, given the fact that delta G is -60 kJ/mol.
d. The activation energy for the reaction is very large; equilibrium has not been reached by 24 hours.
e. An enzyme has shifted the equilibrium toward A.

Explanation / Answer

a. Use the Michaelis Menten equation

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