Hemoglobins from two individuals are compared by electrophoresis and by fingerpr

Question

Hemoglobins from two individuals are compared by electrophoresis and by fingerprinting. Electrophoresis reveals no difference in migration fingerprinting shows an amino acid difference. How is this possible? If the amino acid is substituted with another amino acid that does not drastically alter the tertiary structure of the protein, there is a chance that factors that influence electrophoretic mobility (primarily net charge) will not be altered. If the amino acid is substituted with an amino acid of like charge and similar structure, there is a chance that factors that influence electrophoretic mobility (primarily net charge) will not be altered. If the amino acid is substituted with an amphiphilic amino acid, there is a chance that factors that influence electrophoretic mobility (primarily net charge) will not be altered. If the amino acid is substituted with a nonpolar amino acid, there is a chance that factors that influence electrophoretic mobility (pr net charge) will not be altered.

Explanation / Answer

B. If the amino acid is substituted with an amino acid of like charge and similar structure, there is chance that the factors affecting electrophoretic mobility will not be altered.

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