What prevents oocyte to enter M phase? I am working on a meiotic cell division u

Question

What prevents oocyte to enter M phase?

I am working on a meiotic cell division using Xenopus oocyte. I have taken some genomic DNA and after shearing it through a needle, coated a 100 nm beads to act as chromosome to see what would happen if I have extra chromosome in oocyte.

when coated beads are injected into germinal vesicle of oocyte, and activate oocyte with progesterone, oocyte does not enter M phase. While, if I had uncoated beads, oocyte could go to M phase.

1-I want to know what is going on by drawing the pathway.?

2-what I may do to allow entering to M phase in case of coated beads?

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What is effect of loss of negative regulators of signal transduction on uncontrolled cell proliferation?

How loss of negative regulators of signal transduction result in uncontrolled cell proliferation in a cell that require a mitogen such as EGF to enter cell cycle?

Explanation / Answer

1. If DNA is physically damaged, protein p53 activates genes that either stop cell cycle until damage is repaired and thats why oocyte doesnt enter M phase. Genomic DNA - sheared - coated onto beads- germinal vesicle - added progesterone.

2. If DNA is not damaged then it allows entering to M phase.

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